Bacteriocin Typing for Clinical Isolates of Enterobacter cloacae

Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae

Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78...

Purification and genetic determination of bacteriocin production in Enterobacter cloacae.

Enterobacter cloacae (strain DF13) was found to produce a bacteriocin which could be induced by mitomycin C. In the supernatant fluid of the induced culture phagelike particles were found. The bacteriocin was partially purified from induced cultures by ammonium sulfate precipitation and gel-filtration on Sephadex G-150. Ultraviolet-absorbing material was eluted from the Sephadex column in three...

Bacteriocin typing of streptococcal isolates from endocarditis.

A typing system based on bacteriocin production and sensitivity for Streptococcus sanguis strains was devised. Bacteriocin producer strains were grown (37degrees C) anaerobically on brain heart infusion-yeast extract agar for 18 h. Bacteriocin indicator strains were suspended to molten brain heart infusion-yeast extract agar and then overlayed onto the producer strain. After an additional 18 to...

Gene Mutations Responsible for Overexpression of AmpC -Lactamase in Some Clinical Isolates of Enterobacter cloacae

Gene mutations responsible for overexpression of AmpC beta-lactamase in some clinical isolates of Enterobacter cloacae.

AmpC regulatory genes in 21 ceftazidime-resistant clinical isolates of Enterobacter cloacae (MICs of > or = 16 microg/ml) were characterized. All isolates exhibited AmpC overproduction due to AmpD mutation. Additionally, we found two AmpR mutants among the isolates. This is the first report of chromosomal ampR mutation in clinical isolates of E. cloacae.